This book constitutes the referred proceedings of the 5th International Conference on Machine Learning for Cyber Security, ML4CS 2023, held in Yanuca Island, Fiji, during December 4-6, 2023.The 11 full papers presented in this book were carefully reviewed and selected from 35 submissions. They cover a variety of topics, including cybersecurity, AI security, machine learning security, encryption, authentication, data security and privacy, cybersecurity forensic analysis, vulnerability analysis, malware analysis, anomaly and intrusion detection.