This book constitutes the refereed proceedings of the 25th International Conference on Applications of Evolutionary Computation, EvoApplications 2023, held as part of Evo*2023, in April 2023, co-located with the Evo*2023 events EuroGP, EvoCOP,  and EvoMUSART. The EuroGP focused on the technique of genetic programming, EvoCOP targeted evolutionary computation in combinatorial optimization, and EvoMUSART was dedicated to evolved and bio-inspired music, sound, art, and design. The EvoApplications 2023 presents papers on the different areas: Analysis of Evolutionary Computation Methods: Theory, Empirics, and Real-World Applications, Applications of Bio-inspired Techniques on Social Networks, Evolutionary Computation in Edge, Fog, and Cloud Computing, Evolutionary Computation in Image Analysis, Signal Processing, and Pattern Recognition and others.